Fig. 7: RORC was acetylated and deacetylated by p300 and HDAC1 or SIRT3. | Cell Death & Disease

Fig. 7: RORC was acetylated and deacetylated by p300 and HDAC1 or SIRT3.

From: Intratumoral Brevibacillus parabrevis enhances antitumor immunity by inhibiting NK cell ferroptosis in hepatocellular carcinoma

Fig. 7: RORC was acetylated and deacetylated by p300 and HDAC1 or SIRT3.

A Mass spectrometry showing proteins bound to RORC in NK-92 cells. B Immunoblotting analysis of input and protein-antibody complexes pulled down with anti-Myc antibody from NK-92 cells transfected with Myc-tagged RORC and HA-tagged acetyltransferases (n = 3). C RORC acetylation was detected upon overexpression of acetyltransferases in NK-92 cells (n = 3). D Immunoblotting showing the effects of p300 inhibitors A485 and B026 on RORC acetylation in NK-92 cells (n = 3). Immunoblotting showing the effect of knockdown (E) or overexpression (F) of p300 on RORC acetylation in NK-92 cells (n = 3). G Immunoblotting analysis of input and protein-antibody complexes pulled down by anti-Myc antibody from NK-92 cells transfected with Myc-tagged RORC and HA-tagged p300 in the presence or absence of the HDAC inhibitor TSA or the SIRT inhibitor NIC (n = 3). H Immunoblotting analysis of input and protein-antibody complexes pulled down by anti-Myc antibody from NK-92 cells transfected with Myc-tagged RORC, HA-tagged p300, and Flag-tagged HDACs (n = 3). I RORC acetylation was detected upon HDAC overexpression in NK-92 cells (n = 3). J Immunoblotting showing the effect of HDAC1 inhibitor SB-429201 on RORC acetylation in NK-92 cells (n = 3). Immunoblotting showing the effect of knockdown (K) or overexpression (L) of HDAC1 on RORC acetylation in NK-92 cells (n = 3). M Immunoblotting analysis of input and protein-antibody complexes pulled down by anti-Myc antibody from NK-92 cells transfected with Myc-tagged RORC, HA-tagged p300 and Flag-tagged SIRTs (n = 3). N RORC acetylation upon SIRT overexpression in NK-92 cells (n = 3). O Immunoblotting showing the effect of SIRT3 inhibitor Chlopynostat on RORC acetylation in NK-92 cells (n = 3). Immunoblotting showing the effect of knockdown (P) or overexpression (Q) of SIRT3 on RORC acetylation in NK-92 cells (n = 3). R Diagram of the mechanism by which B. parabrevis improved NK cell antitumor immunity.

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