Fig. 3: Metrnl deficiency aggravates CCl4-induced liver fibrosis. | Cell Death & Disease

Fig. 3: Metrnl deficiency aggravates CCl4-induced liver fibrosis.

From: Cellular crosstalk mediated by Meteorin-like regulating hepatic stellate cell activation during hepatic fibrosis

Fig. 3

Serum AST (A) and ALT levels (B) in WT and Metrnl−/− mice induced by CCl4 for 8 weeks (n = 6 mice per group). C The mRNA expression of α-SMA, Col1, Col3, and Col4 in the liver from WT and Metrnl−/− mice induced by CCl4 (n = 6 mice per group). D Representative Western blot images and quantification analysis of FN, Col4, and α-SMA expression in the liver from WT and Metrnl−/− mice induced by CCl4 (n = 3 mice). E Representative H&E staining, Sirus Red staining, IHC staining of α-SMA of liver sections from WT and Metrnl−/− mice induced by CCl4 (n = 6 per group). Scale bar, black, 200 μm. Serum AST (F) and ALT levels (G) in Lrat-WT and Lrat-Metrnl mice induced by CCl4 for 8 weeks (n = 6 mice per group). H Representative H&E staining, Sirus Red staining, IHC staining for α-SMA of liver sections from Lrat-WT and Lrat-Met mice induced by CCl4 (n = 6 per group). Scale bar, black, 200 μm. I Relative mRNA expression of Metrnl and α-SMA in primary HSCs isolated from Lrat-WT and Lrat-Met mice was analyzed after natural differentiation for 7 days, these mRNA levels were normalized to that of GAPDH (n = 3 per group). All data are mean ± SD. **P < 0.01, and ***P < 0.001.

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