Fig. 7: Increased cardiac inflammation and HDAC2 phosphorylation are involved in Asb10 overexpression-induced pathological cardiac remodeling.
From: Asb10 accelerates pathological cardiac remodeling by stabilizing HSP70
A ELISA assay to detect serum HSP70 levels. N = 7 (EV+Sham, Asb10+Sham, EV + TAC) and 10 (Asb10 + TAC). B Relative mRNA expression of Hspa1 and Asb10 as detected by qPCR. N = 7 (EV+Sham, Asb10+Sham, EV + TAC) and 10 (Asb10 + TAC). C Relative mRNA expression of canonical inflammation markers as detected by qPCR. N = 7 (EV+Sham, Asb10+Sham, EV + TAC) and 10 (Asb10 + TAC). D Representative immunohistochemistry staining images showing cardiac inflammation of mouse hearts. The right panel showed the quantifications. Scale bar = 200 μm. N = 7 (EV+Sham, Asb10+Sham, EV + TAC) and 10 (Asb10 + TAC). E Protein expression of pHDAC2S394, HSP70 and HDAC2 in left ventricular tissue of mice, with GAPDH as loading control as determined by western blot. The right panel showed the quantifications. N = 7 (EV+Sham, Asb10+Sham, EV + TAC) and 10 (Asb10 + TAC). F Protein expression of pHDAC2S394, HSP70 and HDAC2 in Asb10OE and HSP70KD NRVMs treated with PE, with GAPDH as loading control as determined by western blot. The right panel showed the quantifications. N = 6. Two-way ANOVA followed by Turkey’s multiple comparisons test (A–E) was conducted. Three-way ANOVA followed by Turkey’s multiple comparisons test (F) was conducted. Data were represented as Mean ± SEM. *P < 0.05, **P < 0.01, ****P < 0.0001, nsP > 0.05.
