Fig. 3: Proteomic and transcriptomic profile perturbations following incubation with EAPB02303.
A Venn diagram showing the proteins differentially expressed or phosphorylated proteins (q < 0.05, logFC > 1.5) in Pancpec and CFPAC-1 cells after incubation with EAPB02303 (5xIC50) for 24 h compared with untreated controls (n = 3). B Heatmap showing the differentially expressed or phosphorylated proteins in the two cell lines after incubation with EAPB02303. C Venn diagram showing the Gene Ontology (GO) terms significantly enriched (q < 0.05) in the RNA-seq data of Pancpec and CFPAC-1 cells incubated with EAPB02303 (5xIC50) for 6 h compared with untreated controls (n = 3). The enrichment analysis was performed using GSEA and the GO Biological Process category from the Molecular Signatures Database (MSigDB). Heatmap (lower panel) of the GO terms significantly enriched in Pancpec and CFPAC-1 cells after incubation with EAPB02303. Many of the GO terms enriched in both cell lines were related to microtubule-based process. D Enrichment plots of the GO term “microtubule-based movement” in CFPAC-1 (upper panel) and Pancpec (lower panel) cells. E Volcano plot of the RNA-seq data of CFPAC-1 cells incubated or not with EAPB02303 (5xIC50 for 6 h) showing the high number of tubulin-encoding genes among the downregulated genes (q < 0.01, logFC > 1).
