Fig. 6: EAPB02303 is rapidly converted into EAP04303 in cells by COMT, an enzyme associated with poor prognosis in PDAC. | Cell Death & Disease

Fig. 6: EAPB02303 is rapidly converted into EAP04303 in cells by COMT, an enzyme associated with poor prognosis in PDAC.

From: Inhibiting microtubule polymerization with EAPB02303, a prodrug activated by catechol-O-methyl transferase, enhances paclitaxel effect in pancreatic cancer models

Fig. 6

Representative MRM chromatograms of EAPB02303 (307.2 → 291.2), EAPB04303 (321.2 → 306.0), and internal standard (IS) (277.1 → 117.1) in (A) untreated Pancpec cells, (B) Pancpec cells after incubation with 100 nM EAPB02303 after 60 min, and (C) Pancpec COMT−/− cells after incubation with 100 nM EAPB02303 (D) Patients survival curves in function of COMT expression level (high/low) in pancreatic cancer samples. RNA-seq and survival data were from the TCGA database and were downloaded from the Human Protein Atlas. E COMT expression levels in normal pancreas and primary pancreatic cancer tissue samples from the TCGA and GTEx databases. Error bars represents mean ± SD. **** Two tailed unpaired t-test p-values are reported ****p < 0.0001 ***p < 0.001, **p < 0.01, *p < 0.05. ns: not significant.

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