Fig. 1: IAV infection promotes GSDME cleavage in human bronchial epithelial cells.
From: Gasdermin E deficiency limits inflammation and lung damage during influenza virus infection
Human normal bronchial epithelial HBEC3-KT cells were infected with human IAV HKx31 (HK; H3N2), Brazil/78 (BR; H1N1), Solomon Islands/06 (SI; H1N1), Tasmania/09 (Tas; pandemic H1N1), or Perth/09 (Perth; H3N2) at a multiplicity of infection of 3 for 24 h. Uninfected (UN) cells were included for comparison. A Immunoblot of GSDME, caspase-3, and tubulin protein in cell lysates (top) and cell supernatants (bottom). Arrows indicate full length (FL) and cleaved N-terminal p30 subunit of GSDME, as well as caspase-3 precursor (pro) and cleaved p17 and p19 subunits. * Indicates a non-specific band. Data are representative of three independent experiments. Levels of B LDH (OD; optical density), C IL-1β, and D IL-18 in cell supernatants, determined by colorimetric assay or ELISA. B–D Data are shown as experimental replicates that are representative of three independent experiments. Data are expressed as mean ± SD. *P < 0.05, **P < 0.01, ****P < 0.0001, one-way ANOVA.
