Fig. 2: GC cell-derived exosomes promoted the mesenchymal transition of mesothelial cells in vitro. | Cell Death & Disease

Fig. 2: GC cell-derived exosomes promoted the mesenchymal transition of mesothelial cells in vitro.

From: Tumor exosomal circPTBP3 drives gastric cancer peritoneal metastasis via mesothelial-mesenchymal transition

Fig. 2: GC cell-derived exosomes promoted the mesenchymal transition of mesothelial cells in vitro.The alternative text for this image may have been generated using AI.

A Mesothelial cells were co-cultured with exosomes after 0, 24, 48, and 72 h. Blue, Hoechst33342, nucleus of mesothelial cells; green, PKH67, MGC803 exosomes; red, phalloidin. Scale bars, 20 μm. B Microscopic morphology of mesothelial cells. Bar, 40 μm. C Detection of epithelial and mesenchymal proteins (ZO-1, FSP1, α-SMA, Collagen I, and Fibronectin) in mesothelial cells co-cultured with exosomes by western blot analysis. D, E Observation and statistics of mesothelial cells migrating into the bottom surface of the chamber. Scale bars, 100 μm. F, G Observation and statistics of mesothelial cells invading the bottom surface of the chamber. Scale bars, 100 μm. H, I Observation and statistics of GC cells adhering to the mesothelial cell surface. Scale bars, 200 μm. J Immunofluorescence imaging of epithelial and mesenchymal markers of mesothelial cells. Blue, Hoechst33342, nucleus of mesothelial cells; green, ZO-1; red, Fibronectin. Scale bars, 10 μm. (*p < 0.05; **p < 0.01; ***p < 0.001; ns, no significance).

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