Fig. 7: DMD satellite cells have impaired autophagy and senescence dynamics during regeneration. | Cell Death & Disease

Fig. 7: DMD satellite cells have impaired autophagy and senescence dynamics during regeneration.

From: Muscle stem cells in Duchenne muscular dystrophy exhibit molecular impairments and altered cell fate trajectories impacting regenerative capacity

Fig. 7

A Digital PCR quantification of senescence-associated genes Cdkn1a, Cdkn2a, and Cdkn2b from non-injured (NI), 1 day post-injury (1 DPI) and 3 days post-injury (3DPI) B10 and mdx mice showing dysregulation in mdx (P > 0.05 for effect of strain*DPI, however P < 0.0001, P = 0.0134, and P = 0.0577 for effect of strain alone). B Digital PCR quantification of autophagy-associated genes in NI, 1 DPI, and 3 DPI B10 and mdx satellite cells demonstrating impaired autophagy dynamics in mdx cells during regeneration (Strain*DPI = P < 0.0001, < 0.0001, = 0.0007, < 0.0001, and = 0.0006, respectively). C Representative images of IF staining against MYOG (yellow) and MyHC (magenta) from mdx primary myoblasts treated with an autophagy inhibitor (3MA, 5 mM and H2O control) or autophagy inducer (Tat-D11, 10 µM and Scramble control) for 2 h prior to differentiation for 2 days. D Fusion index of each condition was determined and shows increased differentiation after Tat-D11 treatment. Scalebar = 25 µM, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 (two-way analysis of variance (A, B) and two-tailed unpaired t-test (D)). Data are expressed as mean ± SD.

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