Fig. 2: Inhibition of RNF41 promotes migration of BCa cells. | Cell Death & Disease

Fig. 2: Inhibition of RNF41 promotes migration of BCa cells.

From: Loss of RNF41 promotes bladder cancer metastasis through increasing NUDC stability to enhance tubulin polymerization

Fig. 2

A, B Knockdown efficiency of RNF41 by shRNA, measured via western blot and quantitative RT-PCR (**p < 0.01). C, D Migration abilities of T24 and BIU cells after RNF41 knockdown assessed using a wound healing assay (**p < 0.01). Scale bar, 50 μm. E, F Representative images and quantification of migrated or invading cells in a transwell assay, both without and with matrix penetration (**p < 0.01). Scale bar, 100 μm. G Fluorescence imaging of experimental nude mice injected with T24-Luc cells, comparing fluorescence intensity (**p < 0.01). H Lung imaging and quantification of pulmonary nodules (**p < 0.01). I Hematoxylin and eosin (HE) staining of lungs with metastatic tumors. J Ki67 and RNF41 immunohistochemistry (IHC) staining of lungs with metastatic tumors. Statistical analyses were performed using ANOVA. *p < 0.05; **p < 0.01. Scale bar, 100 μm.

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