Fig. 4: RNF41 regulates the stability of NUDC. | Cell Death & Disease

Fig. 4: RNF41 regulates the stability of NUDC.

From: Loss of RNF41 promotes bladder cancer metastasis through increasing NUDC stability to enhance tubulin polymerization

Fig. 4

A T24 and BIU cells transfected with the specified constructs were treated with or without MG132 for 6 h before collection. RNF41 and NUDC proteins were analyzed using the indicated antibodies. B Left: FLAG-RNF41 plasmid was introduced into T24 and BIU cells, followed by the addition of 50 μg/ml cycloheximide (CHX) at 0, 2, 4, 8, and 12 h. Cells were then harvested, and western blot analysis with NUDC antibodies determined the half-life of NUDC protein. Right: Quantification of NUDC protein levels. C Left: RNF41 shRNA was transfected into T24 and BIU cells for 36 h, followed by treatment with 50 μg/ml CHX at specified intervals (0, 2, 4, 8, 12 h) before collection. The half-life of NUDC protein was assessed via western blot. Right: Quantification of NUDC protein levels. D Expression analysis of RNF41 and NUDC in 10 freshly paired metastatic BCa tissue samples (T) and matched adjacent non-tumor tissues (NT). β-actin served as the loading control. E Representative staining images of RNF41 and NUDC in BCa tissues. Scale bar, 50 μm. F, G Compared to adjacent tissues, NUDC protein levels were elevated in BCa tissues (F), and in paired samples, NUDC was also found to be upregulated in BCa tissues (G). H Correlation analysis between RNF41 and NUDC expression levels in BCa tissues, with statistical analyses performed using the χ² test (P < 0.001). R indicates Pearson’s correlation coefficient.

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