Fig. 6: 643943 exhibits synergistic effect with anti-cancer drugs and effectively sensitizes chemoresistant cancer cells to chemotherapy. | Cell Death & Disease

Fig. 6: 643943 exhibits synergistic effect with anti-cancer drugs and effectively sensitizes chemoresistant cancer cells to chemotherapy.

From: Blocking XIAP:CASP7-p19 selectively induces apoptosis of CASP3/DR malignancies by a novel reversible small molecule

Fig. 6

A, B Combination treatments with 643943 and anti-cancer drugs (STS and doxorubicin) exhibit synergistic effect in MCF-7 cells. Immunoprecipitation/Western blot analysis of XIAP:CASP7-p19 complex and cleaved PARP1 in MCF-7 cells treated without or with indicated compounds. GAPDH was used as a loading control. C Cell survival analysis following paclitaxel treatment. MCF-7 and paclitaxel-resistant MCF-7 (MCF-7/TR) cells were treated with paclitaxel at the indicated concentrations for 48 h. D G2M arrest analysis following combination treatment of 643943 and paclitaxel. 643943 treatment significantly recovers the efficacy of paclitaxel in MCF-7/TR. E MTT assays of MCF-7/TR cells treated with 643943, paclitaxel or combination treatment of 643943 and paclitaxel. Cell death of MCF-7/TR cells were analyzed following treatments of 10 nM paclitaxel, 643943 at the indicated concentrations or combination for 24 h. F–H Western blot analysis of β-catenin, ABC transporters, and GAPDH using their specific antibodies in MCF-7/TR treated with DMSO, 5 µM 643943 or 10 µM CASP7 inhibitor (MPS). GAPDH was used as a loading control. A–H Data were obtained from three independent experiments.

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