Table 1 Key reagents and resources used in this study.
From: KDM4B enhances immune surveillance via demethylating cGAS
Reagent or resource | Source | Identifier |
|---|---|---|
Anti-TREX1 | Santa Cruz | Cat# sc-271870 |
Anti-JMJD2B (D7E6) | Cell Signaling Technology | Cat# 8639 |
Anti-phospho-TBK1 (Ser172) (D52C2) XP Rabbit mAb | Cell Signaling Technology | Cat# 5483 |
Anti-phospho-STING (Ser365) (D8F4W) Rabbit mAb | Cell Signaling Technology | Cat# 72971 |
Anti-STING (D2P2F) Rabbit mAb | Cell Signaling Technology | Cat# 13647 |
Anti-TBK1 (D1B4) Rabbit mAb | Cell Signaling Technology | Cat# 3504 |
Anti-GAPDH (mouse) | Proteintech | Cat# 60004-1 |
Anti-mouse cGAS (D3O8O) | Cell Signaling Technology | Cat# 31659 |
Anti-K350me Rabbit antibody | This paper | N/A |
Anti-Histone H3 | Cell signaling technology | Cat# 4499 |
Anti-CREB | Cell signaling technology | Cat# 9197 |
InVivoPlus anti-mouse PD-1 (CD279) | Bio X Cell | Cat# BE0146 |
InVivoPlus rat IgG2a isotype | Bio X Cell | Cat# BE0089 |
KDM4 subfamily inhibitors | ||
JIB-04 | Selleck | Cat#S7281 |
ML324 | Selleck | Cat#S7296 |
CPI-455 HCl | Selleck | Cat#S8287 |
Daminozide | Selleck | Cat#S4800 |
GSK-LSD1 | ApexBio | Cat#1401966-69-5 |
GSK J1 | ApexBio | Cat#1373422-53-7 |
Chemicals, kit, medium | ||
2’3’-cGAMP | InvivoGen | tlrl-nacga23 |
HT-DNA | This paper | N/A |
Chaetocin | Selleck | Cat#S8068 |
polybrene | Sigma | Cat#107689 |
puromycin | Yeasen Biotech | Cat#60210ES60 |
HISTOPAQUE-1077 | Sigma-Aldrich | Cat#10771 |
Subcellular Protein Fractionation kit | Thermo Fisher Scientific | Cat#78840 |
Amino acids l-methionine | Sangon Biotech | Cat# 63-68-3 |
Methionine-depleted DMEM medium | Shanghai Duoning Biotechnology | Cat# B001-003 |
