Fig. 5: Transcriptome analysis reveals that IL-1β is a downstream target affected by AK4. | Cell Death & Disease

Fig. 5: Transcriptome analysis reveals that IL-1β is a downstream target affected by AK4.

From: AK4 promotes nasopharyngeal carcinoma metastasis and chemoresistance by activating NLRP3 inflammatory complex

Fig. 5

Heat-map analysis of 41 downregulated genes screened out between S18 NC versus S18 shAK4#1, S18 shAK4#2 cells and 5–8 F NC versus 5–8 F shAK4#1, 5–8 F shAK4#2 cells (A). Kyoto Encyclopedia of Genes and Genomes pathway determined the MAPK signaling pathway, NOD-like receptor signaling pathway, and cytokine receptor interaction gene sets, which were significantly enriched in DEGs regulated by AK4 (B). Venn diagram analysis shows that IL-1β is a key molecule in the above three signaling pathways (C). The mRNA expression level of IL-1β in AK4 knockdown cells was significantly decreased (D). The expression levels of IL-1β in the conditioned medium of AK4 knockdown cells was significantly decreased (E). Data are represented as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001; Student’s t test.

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