Fig. 5: YAP deletion in astrocytes suppressed astrocytic activation, and inhibited the gain of stemness in astrocytes induced by IS in mice.

A Double immunostaining of Nestin (green) and GFAP (red) in the cortex of YAPf/f and YAPGFAP-CKO mice at 3rd day after IS. B Quantification of the density of Nestin+ and GFAP+ cells as shown in (A) (n = 6 mice per group). C Double immunostaining of BLBP (green) and GFAP (red) in the cortex of YAPf/f and YAPGFAP-CKO mice at 3rd day after IS. D Quantification of the density of BLBP+ and GFAP+ cells as shown in (C) (n = 6 mice per group). E Double immunostaining of Sox2 (green) and GFAP (red) in the cortex of YAPf/f and YAPGFAP-CKO mice at 3rd day after IS. F Quantification of the density of Sox2+ and GFAP+ cells as shown in (E) (n = 6 mice per group). G Double immunostaining of Sox9 (green) and GFAP (red) in the cortex of YAPf/f and YAPGFAP-CKO mice at 3rd day after IS. H Quantification of the density of Sox9+ and GFAP+ cells as shown in (G) (n = 6 mice per group). Scale bars: 20 μm. Data were analyzed by unpaired t-test analysis (B, D, F, H). **P < 0.01, Mean ± SEM.