Fig. 1: aSVZ-NSCs undergo fast activation and niche depletion over time in TKO but not DKO mice. | Cell Death & Disease

Fig. 1: aSVZ-NSCs undergo fast activation and niche depletion over time in TKO but not DKO mice.

From: Rb substantially compensates for the double loss of p130 and p107 in adult but not embryonic neural stem cell lineages

Fig. 1

A Experimental design illustrating tamoxifen treatment and collection periods of adult mice carrying inducible and compound deletion(s) in pocket proteins. Immunohistochemistry (IHC) on brain sagittal sections in the aSVZ at 4wpt and 16wpt in THC (B, B’, E, E’), DKO (C, C’, F, F’), and TKO (D, D’, G, G’) mice. Insets in (B’–G’) show higher magnification images of the boxed regions inside the aSVZ. Note the lateral ventricle’s enlargement and niche depletion in TKO brains at 16wpt. IHC in the corpus callosum (H, I) and striatum (J, K) at 4wpt showing vimentin-positive progeny with astrocyte morphology derived from aSVZ-NSCs, including some progenitors co-expressing YFP and/or Nestin. Insets in (H–J”’) are higher magnification images of the boxed regions. I–K Higher magnification images of dashed boxes in (H”’, K”’). L Quantification of cell counts in the aSVZ showing significant increase in (Nestin + YFP+) and (Nestin + YFP + GFAP+) cell populations in TKO and DKO compared with THC at 4wpt, followed by gradual decrease and stem cell pool depletion in TKO brains (but not DKO) by 16wpt. Cx cortex, LV lateral ventricle, aSVZ adult subventricular zone. Scale bars, (B–G’): 100 µm, (H–K): 50 µm. Error bars, mean ± SD. Unpaired 2-tailed Student’s t-test; *p < 0.05, **p < 0.01, ***p < 0.001. n = 3 biological replicates.

Back to article page