Fig. 5: Loss of RRAGD inhibits melanoma progression and LINC00622 represses autophagic cell death. | Cell Death & Disease

Fig. 5: Loss of RRAGD inhibits melanoma progression and LINC00622 represses autophagic cell death.

From: LINC00622 transcriptionally promotes RRAGD to repress mTORC1-modulated autophagic cell death by associating with BTF3 in cutaneous melanoma

Fig. 5

A RRAGD RNA expression was detected by qPCR after knockdown of RRAGD by RNA interference in SK-MEL-28 melanoma cells. Measurement of cell proliferation by CCK-8 assay (B), colony formation assay (C), Transwell migration assay (D) were performed. E RRAGD, p-mTOR, p-S6K, LC3B and P62 were detected by Western blot in SK-MEL-28 melanoma cells after RRAGD depletion. F Sytox green staining was performed to detect the major type of cell death induced by LINC00622 depletion in melanoma cells together with 3-MA, MYH1485, Z-VAD-FMK or Nec-1 treatments. Scale bar, 100 μM. G Evaluate the role of LINC00622 in repressing Rapamycin-induced cell death in melanoma cells by Sytox green staining. Scale bar, 100 μM. H Transmission electron microscopy (TEM) was performed to detect the type of cell death in melanoma cells. Blue arrows indicate autophagosomes. Typical features of autophagic cell death includes the formation of empty vacuoles (EVs) indicated by black arrows and perinuclear space (PNS) showing the separation of outer nuclear membrane (ONM) and inner nuclear membrane (INM) indicated by red arrows. Scale bar, 2 μM.

Back to article page