Fig. 2: Knockdown of circ0515 inhibits proliferation, cell migration and tumor growth in lung cancer. | Cell Death & Disease

Fig. 2: Knockdown of circ0515 inhibits proliferation, cell migration and tumor growth in lung cancer.

From: Circ0515 reprogramming mitochondrial succinate metabolism and promotes lung adenocarcinoma progression through regulating SDHB

Fig. 2

A–D CCK8 experiments detect the effect of circ0515 knockdown and overexpression on lung cancer cell proliferation. E, F Clonogenic assays detect the effect of circ0515 knockdown and overexpression on lung cancer cell colony formation ability. G, H Transwell assays detect the effect of circ0515 knockdown and overexpression on lung cancer cell migration ability. I Western blot experiments detect the effect of circ0515 knockdown on EMT-related proteins in lung cancer cells. J–L Xenograft experiments in nude mice to assess the impact of the circ0515 knockdown on lung cancer tumor growth. K shows the tumor weight, L shows the tumor volume, (n = 3 mice per group), PCDH PCDH-Vec, circ0515 Over circ0515 Overexpression, sh#1 circ0515 shRNA#1, sh#2 circ0515 shRNA#2. NC Negative control, mimics miR-328-3p mimics, ns means not significant, *P < 0.05, **P < 0.01, ***P < 0.001 (Student’s t-test).

Back to article page