Fig. 4: circ0515 activiting the PI3K-AKT signaling pathway. | Cell Death & Disease

Fig. 4: circ0515 activiting the PI3K-AKT signaling pathway.

From: Circ0515 reprogramming mitochondrial succinate metabolism and promotes lung adenocarcinoma progression through regulating SDHB

Fig. 4

A Analysis of the correlation between miR-328-3p and its target gene expression in lung adenocarcinoma using the TCGA database. B qPCR analysis of changes in downstream target gene expression following miR-328-3p overexpression. C Evaluation of the prognostic value of miR-328-3p target genes in LUAD using the TCGA database. D, E Dual-luciferase reporter assays to assess the binding interaction between miR-328-3p and its target genes in A549 cells. F qPCR analysis of changes in downstream target gene expression following inhibition of endogenous miR-328-3p. G, H qPCR experiments to determine the expression of miR-328-3p target genes after overexpression or knockdown of circ0515 in lung cancer cells. I, J Cell proliferation rescue assays involving circ0515/miR-328-3p/YWHAZ interactions. K Western blot experiments to detect the effect of circ0515 knockdown on PI3K-AKT signaling pathway-related proteins in lung cancer cells. L, M ELISA experiments to measure PI3K production following circ0515 knockdown or overexpression in lung cancer cells. PCDH PCDH-Vector control, circ0515 Over circ0515 overexpression, sh#1 circ0515 shRNA#1, sh#2 circ0515 shRNA#2. YWHAZ YWHAZ overexpression. ns means not significant, *P < 0.05, **P < 0.01, ***P < 0.001 (Student’s t-test).

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