Fig. 4: OTUD6B promotes CD8+ T cell infiltration by upregulating CXCL11. | Cell Death & Disease

Fig. 4: OTUD6B promotes CD8+ T cell infiltration by upregulating CXCL11.

From: ATRA upregulates OTUD6B to recruit CD8+ T cells to suppress colorectal liver metastasis by stabilizing DDX5/STAT3/CXCL11 axis

Fig. 4: OTUD6B promotes CD8+ T cell infiltration by upregulating CXCL11.

A The relative mRNA levels of chemokines from OTUD6B-overexpression (left) or knockdown (right) HCT116 and SW480 cells. B, C Cellular supernatant, CXCL11, was detected using an ELISA kit from human CRC cells (HCT116 and SW480). D The relative mRNA levels of Cxcl11 from Otud6b overexpression (left) or knockdown (right) MC38 cells. E Cellular supernatant Cxcl11 was detected by ELISA kit from mouse CRC cells (MC38). F The relative mRNA levels of Otud6b (left) and Cxcl11 (right) from liver tissues injected with MC38-Otud6b-overexpression cells in C57BL/6J mice (n = 4). G Serum Cxcl11 was detected using an ELISA kit from (F). H The relative mRNA levels of Otud6b (left) and Cxcl11 (right) from liver tissues injected with MC38-Otud6b-knockdown cells in C57BL/6J mice (n = 4). I Serum Cxcl11 was detected using an ELISA kit from (H). J Representative pictures and quantitative results of liver metastatic nodules from C57BL/6J mice intraperitoneally injected with or without AMG487 (CXCR3 inhibitor) treatment (n = 5). K Representative pictures and quantitative results of H&E staining of liver tissue sections from (J). Data are expressed as mean ± SEM. A two-sided Student’s t test was used for the statistical analysis. *P < 0.05; **P < 0.01; ***P < 0.001; ns not significant.

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