Fig. 5: OTUD6B stabilizes DDX5 protein expression.

A There were 558 proteins interacting with OTUD6B in HCT116 cells through IP-MS and 52 possible transcription factors to transcribe CXCL11 from hTFtarget. Take the intersection of IP-MS and transcription factor prediction. B Survival analysis between patients with DDX5 high- and low-expression COAD and READ using kmplot database. C The relative mRNA level of DDX5 from OTUD6B-overexpression or control HCT116 cells. D HCT116 cells were transfected with the indicated plasmids, followed by treatment with MG132 (5 µM) or not for 12 h before collection. E HCT116 cells were transfected with the indicated plasmids for 48 h. Cells were subsequently treated with cycloheximide (CHX, 50 µM) and collected for immunoblot analysis at the indicated time points. F, G HCT116 cells transfected with indicated plasmids for 48 h. Cells were lysed with IP buffer and subsequently analyzed using co-IP with Flag or Myc antibody followed by Western blot. H HCT116 cells were transfected with the indicated plasmids, followed by treatment with MG132 (5 µM) for 12 h before collection. The lysates were incubated with Myc antibody and then subjected to immunoblotting. I Representative images of IHC staining of OTUD6B and DDX5 expression in colorectal tumor (Co-T) and adjacent normal (Co-N) specimens (left). Representative images of IHC staining of OTUD6B and DDX5 expression in liver tumor (Li-T) and adjacent normal (Li-N) specimens (right). Scale bar = 200 µm. Data are expressed as mean ± SEM. A two-sided Student’s t-test was used for the statistical analysis. ns, not significant.