Fig. 7: IFFO1 was downregulated in lung cancer and associated with tumor progression.

A IFFO1 expression between tumor tissues and normal tissues in the TCGA lung cancer dataset. B Kaplan–Meier survival analysis of overall survival. Patients were assigned to the high and low expression groups according to the IFFO1 expression level. C Microarray analysis of IFFO1 expression between tumor tissues and normal tissues from the GSE18842 dataset. D IFFO1 expression profile between tumor tissues and adjacent normal tissues from the GSE19188 dataset. E Comparison of IFFO1 expression between tumor samples and non-tumor lung samples from the GSE30219 dataset. F Comparison of IFFO1 expression between tumor samples with or without relapse in the GSE30219 dataset. G IQGAP3 expression between tumor tissues and normal tissues in the TCGA lung cancer dataset. H Kaplan–Meier survival analysis of overall survival according to the IQGAP3 expression level. I Correlation analysis of expression levels between IFFO1 and IQGAP3 from the GSE30219 dataset. J Immunohistochemical staining of IFFO1 in tumor tissues and adjacent normal tissues from tissue microarray chips (Shanghai Outdo Biotech Company). Scale bar = 50 μm. K Immunohistochemical staining and IRS (immunoreactivity score) of IFFO1 in primary tumors without metastasis and tumors with lymph node metastasis. Each group comprises ten tissue sections, from which 20 to 30 regions are randomly selected from each section. These regions are independently evaluated by two pathologists. The red arrow indicates the positive expression of IFFO1 in lung epithelial cells. Scale bar = 50 μm. ****p < 0.0001.