Fig. 1: Melanoma cells transdifferentiate into neuron-like cells.
From: Direct transdifferentiation of tumorigenic melanoma cells induces tumor cell reversion
A Schematic overview of the 4-factor (4F) tumor cell transdifferentiation procedure. B, C Verification of the ectopic overexpression of the factors used for the transdifferentiation of cells into neurons. D Comparison of the morphologies of transdifferentiating and parental melanoma cells. After 14 days of treatment with doxycycline and enrichment by palbociclib treatment, neuron-like cells with round somata and long processes were visible. E The expression of neuronal markers was elevated at the RNA level after treatment with doxycycline for 7 days. F Expression of neuronal markers could also be demonstrated at the protein level via immunostaining 7 days after induction with doxycycline.
