Fig. 5: Increased sensitivity of transdifferentiated cells to radiotherapy.
From: Direct transdifferentiation of tumorigenic melanoma cells induces tumor cell reversion
A Quantification of the percentage of apoptotic HT144 and MA1 melanoma cells 96 h after irradiation with 3 Gy. Early apoptotic cells (Annexin V⁺/DAPI−) appear in the lower right quadrant, indicating phosphatidylserine externalization with an intact membrane. Late apoptotic cells (Annexin V⁺/DAPI⁺) are in the upper right quadrant, characterized by both phosphatidylserine exposure and membrane permeability. Necroptotic cells (Annexin V−/DAPI⁺) are in the upper left quadrant, showing membrane rupture without phosphatidylserine externalization. The percentage of apoptotic and dead cells was much higher among the transdifferentiated cells in comparison to the parental melanoma cells. B gH2AX analysis 0.5, 1, 6, or 24 h after single treatment of HT144 and MA1 melanoma cells with 3 Gy radiation.
