Fig. 3: Knockdown of HOXC8 induces caspase-1-mediated pyroptosis in LUAD cells. | Cell Death & Disease

Fig. 3: Knockdown of HOXC8 induces caspase-1-mediated pyroptosis in LUAD cells.

From: HOXC8 impacts lung tumorigenesis by preventing pyroptotic cell death through the suppression of caspase-1 expression

Fig. 3

A A549 cells were infected with lentiviral vector containing Scrambled sequence (control) or HOXC8 shRNAs for 2 days and then cultured in the presence of vehicle (DMSO) or 10 µM inhibitor of each individual caspase for 2 days followed by an MTT assay to analyze cell viability. Data are means ± SEM. ***P < 0.001. B A549 and H460 cells were lentivirally transduced with either Scrambled sequence (control) or HOXC8 shRNAs for 3 days followed by analysis of caspase-1 activity. Data are means ± SEM. **P < 0.01. C A549 and H460 cells were lentivirally transduced with either Scrambled sequence (control) or HOXC8 shRNAs for 3 days and conditioned media were then collected to measure LDH activity. Data are means ± SEM. n = 3. ***P < 0.001. D A549 and H460 cells were lentivirally transduced with either Scrambled sequence (control) or HOXC8 shRNAs for 3 days and conditioned media were then collected to measure the level of IL-1β. Data are means ± SEM. **P < 0.01. E A549 and H460 cells were lentivirally transduced with either Scrambled sequence (control) or HOXC8 shRNAs for 2 or 4 days followed by western blotting to detect gasdermin D (GSDMD) and βActin with the respective antibodies. F A549 cells were lentivirally transduced with either Scrambled sequence (control) or HOXC8 shRNAs for 2 days and then culture in the presence of vehicle (DMSO) or 10 µM disulfiram for another 2 days, followed by an MTT assay to analyze cell viability. Data are means ± SEM. **P < 0.01; ***P < 0.001.

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