Fig. 6: HOXC8 recruits HDAC1 to CASP1 promoter to suppress caspase-1 expression. | Cell Death & Disease

Fig. 6: HOXC8 recruits HDAC1 to CASP1 promoter to suppress caspase-1 expression.

From: HOXC8 impacts lung tumorigenesis by preventing pyroptotic cell death through the suppression of caspase-1 expression

Fig. 6

A H460 cells were immunoprecipitated with either HDAC1 or HOXC8 antibody and the immunoprecipitates were subjected to western blot to detect HOXC8 or HDAC1, respectively. B H460 cells were subjected to immunofluorescence staining to detect HOXC8 and HDAC1. Data are the representative of six independent experiments. C Schematic diagram of GST-tagged HDAC1 deletion constructs. D A549 cell lysates were incubated with GST beads containing various HDAC1 fragments on ice for 2 h. After 4 washes, beads were boiled and then subjected to western blot analysis to detect HOXC8. E A549 cells were subjected to ChIP with IgG or rabbit anti-HOXC8 polyclonal antibody followed by qPCR to analyze the occupancy of HOXC8 in P1, P2, and P3 regions of CASP1 promoter. Data are means ± SEM. **P < 0.01. F A549 and H460 cells were lentivirally transduced with either Scrambled sequence (control) or HOXC8 shRNAs for 3 days and then subjected to HDAC1 ChIP followed by qPCR to analyze HDAC1 occupancy in the indicated region of CASP1 promoters. Data are means ± SEM. ***P < 0.001.

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