Fig. 1: ILF3 is decreased in RSL3-mediated ferroptosis. | Cell Death & Disease

Fig. 1: ILF3 is decreased in RSL3-mediated ferroptosis.

From: ILF3 promotes colorectal cancer cell resistance to ferroptosis by enhancing cysteine uptake and GSH synthesis via stabilizing SLC3A2 mRNA

Fig. 1: ILF3 is decreased in RSL3-mediated ferroptosis.The alternative text for this image may have been generated using AI.

A Lipid peroxidation levels in SW620 cells treated with RSL3 (10 μM, 24 h) were detected using a BODIPY (581/591) C11 probe. Scale bar = 10 μm. Red represents reductive status, green represents oxidative status, and blue represents DAPI staining. B MDA levels in SW620 cells treated with RSL3 (10 μM, 24 h) compared to the control group. C Volcano plot showing 10,777 DEGs in SW620 cells after RSL3 treatment (10 μM, 24 h) (|log₂FC | ≥ 1, p-value ≤ 0.05). D Heatmap displaying the top 10 RBPs with significant expression differences and several related positive genes ACSL4, HMOX1, TFRC, and FTH1 in SW620 cells. E RT-qPCR analysis of mRNA levels of the top 10 RBPs following RSL3 treatment (10 μM, 24 h). F Western blot analysis of ILF3, GNL3, LARP7, ZFP36, and SYNE1 protein levels in SW620 and DLD-1 cells treated with RSL3 (10 μM, 24 h). G Western blot analysis of ILF3 protein levels in SW620 and DLD-1 cells treated with various concentrations of Erastin. H Western blot analysis of ILF3 protein levels in SW620 and DLD-1 cells subjected to cystine deprivation.

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