Fig. 3: C-ion irradiation induces the IFN-I response in MDA-MB-436. | Cell Death & Disease

Fig. 3: C-ion irradiation induces the IFN-I response in MDA-MB-436.

From: Molecular features of TNBC govern heterogeneity in the response to radiation and autophagy inhibition

Fig. 3

Cells were irradiated with 8 Gy or 3×8 Gy X-rays or 8 Gy C-ions and harvested 72 h after the last irradiation. Cells were treated for 48 h with 1 μM of taxol, 3 μM SAR405 or 200 nM bafilomycin A1. When combined with radiation, SAR405 and bafilomycin A1 were added 72 h before harvesting. A Western blots of MDA-MB-436 upon different treatments. GAPDH served as a loading control. B RT-qPCR analysis of IFNB1, CXCL10, PD-L1 and IL-6 mRNA levels in MDA-MB-436. TBP served as a housekeeping gene. C Nuclear IRF3 analysis by flow cytometry for MDA-MB-436 control cells and after treatment with 8 Gy X-rays or C-ions. Flow cytometry measurements were analyzed with FlowJo_v10.8.1. The bars and error bars represent mean values ± standard deviation. Three biologically independent experiments were performed. P values were calculated with a one-way ANOVA (* ≤ 0.05; **≤ 0.01; ***≤ 0.005, ****≤0.0001).

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