Fig. 2: The formation of migrasomes.
From: Biogenesis, functional roles, and pathological implications of migrasomes
a Nucleation, PIP5K1A converts PI4P to PI(4,5)P2, PI(4,5)P2 interacts with the C-terminus of RAB35, thereby recruiting more RAB35 and integrin α5. b Maturation, cells gradually migrate away, while the anchored SMS2 spots remain on the retraction fibers, becoming the formation site of migrasomes. At the SMS2 spots, ceramide is converted into sphingolipids, thus promoting the growth of migrasomes. c Amplification, TSPAN and other proteins, integrins, cholesterol combine to form TSPAN4-enriched microdomains. d ROCK1 interacts with fibronectin, regulating the formation of migrasomes. e Peptide-modified surfaces promote the formation of longer retraction fibers and more migrasomes by cells. f Cell migration affects the formation of migrasomes by regulating the number and length of RFs.
