Fig. 2: Hematopoiesis-specific Lsd1 knockout displays shorter survival, pancytopenia, increased hematopoietic stem and progenitor cells, and myeloid cells in the bone marrow.

A–M Lsd1^fl/fl mice without Cre served as separate controls in both mouse lines (ko ctr^BMT and ei ctr^BMT). A–C, F–L Statistical analyses of peripheral blood and bone marrow (BM) populations were conducted using Student’s t tests. n = 7–9 per genotype. M Survival analysis was performed using Log-Rank (Mantel-Cox) testing. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. n = 7–9 per genotype. Only statistically significant comparisons are denoted. A White blood cell counts (WBC), B Platelet (PLT) counts, and C hemoglobin (HGB) values of Lsd1 ko^BMT and Lsd1 ei^BMT mice. Histopathological BM slides of D Lsd1 ko ctrl^BMT (left) and Lsd1 ko^BMT (right), as well as E Lsd1 ei ctrl^BMT (left) and Lsd1 ei^BMT (right) femora. 200x magnification (top) and 1000x magnification (down). Sections were stained using hematoxylin and eosin. Frequency of myeloid cells in the BM of Lsd1 ko^BMT and Lsd1 ei^BMT mice by flow cytometry analysis: F CD11b⁺ positive cells, G CD11b⁺ Gr-1^neg, H CD11b⁺ Gr-1^low, I CD11b⁺ Gr-1^high. J–L Hematopoietic stem and progenitor cell (HSPC) frequency of Lsd1 ko^BMT and ei^BMT mice in the BM by flow cytometry analysis: J long-term HSCs (LT-HSC), K short-term HSCs (ST-HSC), and L multipotent progenitors (MPP). M Kaplan–Meier survival curves of Lsd1 ko^BMT and Lsd1 ei^BMT mice.