Fig. 1: Caspase-3 is highly expressed in melanoma cells where it interacts with cytoskeletal proteins.

A Analysis of CASP3 expression in normal human tissues obtained from the GTEx (the Adult Genotype Tissue Expression) Portal. Skin tissue is highlighted. B Analysis of the most frequent mutations occurring in melanoma in the TCGA-SKCM dataset. CASP3 mutation rate is highlighted in orange. C Analysis of caspase expression in 39 melanoma cell lines from the Cancer Cell Line Encyclopedia (CCLE). D Comparison of CASP3 expression in primary (n = 104) and metastatic melanoma (n = 368) in the TCGA-SKCM dataset (p = 2.36 × 10−7). E Clustering based on gene ontology (GO)-based classification of CASP3 interacting proteins identified by mass spectrometry after immunoprecipitation of CASP3-GFP (compared to GFP only) in WM852 cells. F Analysis of the type of protein domains contained in caspase-3 interacting partners in WM852. G Clustering based on the GO-based classification of CASP3-interacting proteins identified after immunoprecipitation of CASP3-GFP in WM793 and WM852 cells.