Fig. 3: Caspase-3 controls melanoma cell motility. | Cell Death & Disease

Fig. 3: Caspase-3 controls melanoma cell motility.

From: Atypical contribution of caspase-3 to melanoma cancer cell motility by regulation of coronin 1B activity

Fig. 3

A Quantification of cell adhesion potential of parental and CASP3-depleted WM793 cells (n = 3, one representative experiment shown, two-way ANOVA statistical test; Statistical significance: ns - P > 0.05; * - P ≤ 0.05; ** - P ≤ 0.01; *** - P ≤ 0.001; **** - P ≤ 0.0001). B Time-lapse cellular tomography in WM793 cells. C Schematic representation of the wound healing assay using the IncuCyte live cell imager, which assesses the migratory ability of melanoma cells to close a wound. Quantification of migration potential in parental and CASP3-knockdown WM793 (D) and WM852 (E) cells through kinetic wound area measurement (n = 3, one representative experiment shown, two-way ANOVA statistical test; Statistical significance: ns - P > 0.05; * - P ≤ 0.05; ** - P ≤ 0.01; *** - P ≤ 0.001; **** - P ≤ 0.0001). F Schematic representation of the invasion assay through matrigel. Measurement of the invasion potential of WM793 (G) and WM852 (H) depleted or not for CASP3 (n = 3, one representative experiment shown, two-way ANOVA statistical test; Statistical significance: ns - P > 0.05; * - P ≤ 0.05; ** - P ≤ 0.01; *** - P ≤ 0.001; **** - P ≤ 0.0001). I Schematic representation of the chemotaxis assay. Measurement of control and CASP3-knockdown WM793 (J) and WM852 cells (K) chemotaxis potential (n = 3, one representative experiment shown, two-way ANOVA statistical test; Statistical significance: ns - P > 0.05; * - P ≤ 0.05; ** - P ≤ 0.01; *** - P ≤ 0.001; **** - P ≤ 0.0001). L The zebrafish model of cancer metastasis. M DiD-labelled CASP3 knockdown and DiO-labelled control WM793 cells were pre-mixed in equal numbers and injected in the zebrafish embryos yolk sac as shown in (L). A representative epifluorescence image of a whole embryo shows perivitelline homing and caudal blood vessels invasion of cancer cells. White arrows point towards invading cells. N Quantification of invaded metastatic cells per zebrafish embryo, (data represent mean with SEM, n = 39 embryos from three independent experiments, t-test, ***p < 0.001).

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