Fig. 3: Detection of tissue biomarkers of cachexia and fibrosis in AT from CRC patients.

AT relative mRNA levels of (A) IL-8, (B) IL-10, (C) IL-15, (D) collagen (left panel), (E) TGF-β. The fold increase of mRNA expression was calculated using the 2−ΔCt method; actin was used as internal control. Data are mean ± standard errors. *p < 0.05 vs HVAT samples. (n = 10 for each group). D, right panel). Western blot analysis for adipose collagen. The histograms show the densitometric analysis. Values are expressed as fold increase. Vinculin was used as internal control. Data are mean ± standard errors. *p < 0.05 vs HVAT samples.