Fig. 2: FLASH RT induces similar levels of lipid peroxidation and ferroptosis with conventional RT in cancer cells.
From: Tissue-specific iron levels modulate lipid peroxidation and the FLASH radiotherapy effect
A, B A549 (A) and MDA-MB-231 (B) cells were irradiated at 10 Gy with conventional or FLASH dose rates with an electron linear accelerator. A significant increase in lipid peroxidation was observed 24 h after RT using C11 BODIPY in all treatment groups. C, D A significant dose-dependent induction of lipid peroxidation was observed after conventional and FLASH RT. E, F Clonogenic survival was determined after conventional or FLASH RT with or without pre-treatment of 20 µM (A549) or 4 µM (MDA-MB-231) Ferr-1. Error bars indicate standard deviation (SD) (n = 3 per group). Statistical tests were performed by One-way ANOVA with Dunnett’s multiple comparisons test (A, B), Two-way ANOVA with Tukey’s multiple comparisons test (C, D), and One-way ANOVA with Tukey’s multiple comparisons test (E, F).
