Fig. 9: Analysis of the synergistic regulatory mechanism of FBP2+sh-HKDC1 on GC.

A Volcano plot and B heatmap generated from the RNA sequencing data of FBP2+sh-HKDC1 and Vector+sh-NC cells, along with C PPI network constructed based on the top 10 significant DEGs. D GO and E KEGG pathway enrichment analysis was performed based on the DEGs. F Differentially expressed transcription factor families and their corresponding gene members. G Impact of FBP2+sh-HKDC1 on the expression levels of key glycolytic proteins in GC cells. H Schematic illustration of the synergistic regulatory mechanism of FBP2 and sh-HKDC1 on glucose metabolism in GC.