Fig. 1: Elevated expression of OTUB1 is associated with poor PC patient prognosis. | Cell Death & Disease

Fig. 1: Elevated expression of OTUB1 is associated with poor PC patient prognosis.

From: The deubiquitylase OTUB1 drives gemcitabine resistance in pancreatic cancer by enhancing pyrimidine metabolism through modulating DHODH mRNA stability

Fig. 1

A TCGA-PAAD database analysis shows the 16 differentially expressed human OTU domain DUBs in PC tissues and their corresponding adjacent normal tissues. *p < 0.05; ***p < 0.001. B Venn diagram showing OTUB1 expression in the PCGA-PAAD, GEO28735, GEO16515, and GEO15471 dataset. C OTUB1 expression levels were significantly higher in the PC tissues compared to the adjacent normal tissues in all four datasets. D qRT-PCR analysis of OTUB1 mRNA expression in PC tumors and paired normal tissues. E Determination (left) and quantitation (right) of OTUB1 protein levels in PC tissues and paired normal tissues by Western blot assay. Tubulin was used as the internal standard. ***p < 0.001. F, G Representative image (left) and quantitation (right) of OTUB1 staining were displayed in PC tissues and paired normal tissues (magnification ×100, inset magnification×400). Scale bar, 50 μm. ***p < 0.001. H, I Kaplan–Meier plots representing probabilities of overall (H) and progression-free survival (I) in 86 PC patients with high- and low-OTUB1 expression.

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