Table 1 Key reagents, resources, and tools used in this study.
REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
Protein ladder | ||
PageRuler™ Prestained | Thermo Scientific | Cat# 26616 |
PageRuler™ Plus | Thermo Scientific | Cat# 26619 |
Antibodies | ||
Anti-β-actin | Cell Signaling Technology | Cat# 4970S |
Anti-MLKL | Millipore | Cat# MABC604 |
Anti-Tubulin | Protein Tech | Cat# HRP-66031-1 |
Anti-rat Alexa Fluor 488 | ThermoFisher Scientific | Cat# A11006 |
Anti-rat-HRP | Invitrogen | Cat# 31470 |
Anti-mouse-HRP | Cell Signaling Technology | Cat# 7074S |
Anti-UCP1 | Bio-Techne | Cat# MAB6158 |
Biological Samples | ||
Fetal bovine serum | Sigma-Aldrich | #F7524 |
Newborn calf serum | Biosera | #CA-1151500 |
Chemicals, Peptides, and Recombinant Proteins | ||
3-isobutyl-1-methyl xanthine (IBMX) | Sigma-Aldrich | #I7018 |
CellTiter-Glo® Luminescent Cell Viability Assay | Promega | # G7570 |
cIAP1/2 inhibitor, BV-6 | MedChemExpress | #HY-16701 |
Collagenase | ThermoFisher Scientific | #17018-029 |
DAPI | Sigma-Aldrich | #D1306 |
Dexamethasone | Sigma-Aldrich | #D4902 |
Insulin | Sigma-Aldrich | #I0516 |
Pan-caspase inhibitor, Z-VAD(OMe)-FMK | MedChemExpress | #HY-16658 |
Rosiglitazone | Sigma-Aldrich | #D2408 |
TNF-α | PeproTech | #315-01 A |
Critical Commercial Assays | ||
Cell Line Nucleofector® Kit V | Lonza | #VCA-1003 |
HCS LipidTOX™ Red Neutral Lipid Stain | ThermoFisher Scientific | #H34476 |
Pierce BCA Protein Assay Kit; | ThermoFisher Scientific | #23225 |
MycoAlertTM PLUS Mycoplasma Detection Kit | Lonza | #LT07-701 |
Nucleospin RNAkit | Macherey-Nagel | #740955 |
Triglyceride Infinity Kit | ThermoFisher Scientific | #TR22421 |
Deposited Data | ||
RNA sequencing | Novogene Europe | GSE201450 |
Experimental Models: Cell Lines | ||
3T3-L1 | ATCC | CL-173 |
Experimental Models: Organisms/Strains | ||
Wild type | Janvier labs | C57BL/6JRj |
Oligonucleotides | ||
Table 2 | ||
Recombinant DNA | ||
Mlkl (BC023755) Mouse Untagged Clone | Origene | #MC206757 |
pSpCas9(BB)-2A-GFP (PX458) | Addgene | plasmid #48138 |
Software and Algorithms | ||
Prism | Graphpad Software | N/A |
