Table 2 List of primer sequences used for Real-time quantitative PCR (RT-qPCR) analysis.
Primer | Sense primer | Antisense primer |
|---|---|---|
Acc | 5’ ATGGGCGGAATGGTCTCTTTC 3’ | 5’ TGGGGACCTTGTCTTCATCAT 3’ |
Adipoq | 5’ GTTCCCAATGTACCCATTCGC 3’ | 5’ TGTTGCAGTAGAACTTGCCAG 3’ |
Bckdhb | 5’ AGTGCCCTGGATAACTCATTAGC 3’ | 5’ GCATCGGAAGACTCCACCAAA 3’ |
Chrebp | 5’ CGGACTCGGATACGGACTTG 3’ | 5’ GAAGTGTCCGCTGTGGATGA 3’ |
Cxcr4 | 5’ TCTATGTGGGCGTCTGGATC 3’ | 5’ CAGGACGAGACCCACCATTA 3’ |
Fabp4 | 5’ AAGGTGAAGAGCATCATAACCCT 3’ | 5’ TCACGCCTTTCATAACACATTCC 3’ |
Foxa1 | 5’ ACATTCAAGCGCAGCTACCC 3’ | 5’ TGCTGGTTCTGGCGGTAATAG 3’ |
Gsta4 | 5’ GCCTGGAGACAACAATCCCA 3’ | 5’ CCGTCCCCTGCCATTAAAGT 3’ |
Hprt1 | 5’ AGTCCCAGCGTCGTGATTAG 3’ | 5’ TTTCCAAATCCTCGGCATAATGA 3’ |
Ip6k2 | 5’ TTCGGACTGTGAACCAAAAAGT 3’ | 5’ TCATGCTCCAAGGGTTATAGTGT 3’ |
Lpin1 | 5’ GGCCCTCAACACCAAAAAGTG 3’ | 5’ CGCTGTGAATGGCCTGAAAAT 3’ |
Mlkl | 5’ TTAGGCCAGCTCATCTATGAACA 3’ | 5’ TGCACACGGTTTCCTAGACG 3’ |
Pparα | 5’ AGAGCCCCATCTGTCCTCTC 3’ | 5’ ACTGGTAGTCTGCAAAACCAAA 3’ |
Pparγ | 5’ GCCAGTTTCGATCCGTAGAA 3’ | 5’ ATTCCTTGGCCCTCTGAGAT 3’ |
Rplp0 | 5’ TCGGGTCCTAGACCAGTGTTC 3’ | 5’ AGATTCGGGATATGCTGTTGGC 3’ |
Sgk1 | 5’ GAGCCGGAGCTTATGAACG 3’ | 5’ AGTGAAAGTCGGAGGGTTTGG 3’ |
Ucp1 | 5’ ACTGCCACACCTCCAGTCATT 3’ | 5’ CTTTGCCTCACTCAGGATTGG 3’ |
Wee1 | 5’ TGGCTGGCTCTGTTGATGAG 3’ | 5’ AGCATCAGCTAAACTCCCACC 3’ |
