Fig. 3: IGF2BP3 mediates lipid metabolism in NSCLC.

A Volcano plot of differentially expressed genes (fold change > 1.5, p < 0.05) in H1299 cells stably expressing shRNAs targeting IGF2BP3 (shIGF2BP3) or scramble control (shCtrl). B Heatmap of differentially expressed genes (fold change > 1.5, p < 0.05) between IGF2BP3-knockdown and control H1299 cells measured by z-score. The color means the z score of cluster analysis (C) KEGG enrichment analysis of differentially expressed genes (fold change > 1.5, p < 0.05) identified by RNA-seq in IGF2BP3-knockdown H1299 cells. The color means −log10 (p value) and the dot size means gene count of different pathways. D Gene Set Enrichment Analysis (GSEA) showing decreased fatty acid metabolism in IGF2BP3-knockdown cells. E, F Principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA) of lipidomics data in IGF2BP3-knockdown H1299 cells. G Volcano plot of differential metabolites (fold change > 1.5, p < 0.05) in IGF2BP3-knockdown H1299 cells. H KEGG enrichment analysis of differential metabolites (fold change > 1.5, p < 0.05) identified by lipidomics in IGF2BP3-knockdown H1299 cells. The color means p value and dot size means gene count of pathways. I Heatmap of differential metabolites (fold change > 1.5, p < 0.05) between IGF2BP3-knockdown and control H1299 cells measured by z-score. The color means the z score of cluster analysis. J, K Neutral lipid levels detected by BODIPY 493/503 staining in IGF2BP3-knockout brain tumors examined by microscope. Representative fluorescent images (J) and quantification (K) are shown. Data are presented as mean ± SD; n = 3. ***P < 0.001 (two-tailed t-test).