Fig. 1: Altered proportions and organisation of immune cells in aged SLOs.

A tSNE plot showing cell origin by colour in young and old LNs. B tSNE plot of cell clusters in LN tissues. C Chart showing the ratios of immune cell subsets in young and old LNs. D UMAP plot showing cell origin by colour in young and old spleen. E UMAP plot of cell clusters in spleen tissues. F Chart showing the ratios of immune cell subsets in young and old spleen. G Percentage of T and B cells in young and old LNs (n = 5 mice/group). H Representative immunostaining of LN for B cells (B220, green) and T cells (CD3, red). Scale bars represent 100 μm (n = 5 mice/group). I Histological sections of LNs with immunostaining for B cells (B220, green) and measurement of the area of B220⁺ B cell follicles and minimum distance to capsule in young and old mice. Scale bars represent 200 μm (n = 5 mice/group). J Percentage of T and B cells in young and old spleen (n = 5 mice/group). K Representative immunostaining of spleen for B cells (B220, green) and T cells (CD3, red). Scale bars represent 100 μm (n = 5 mice/group). P-values were calculated between two groups using an unpaired t-test. ns, not significant; *P < 0.05; **P < 0.01; ***P < 0.001. BC B cell, TC T cell, NK natural killer cell, cDC classical dendritic cell, Mono monocyte, Macro macrophage, pDC plasmacytoid dendritic cell, Neu neutrophil.