Fig. 2: Altered immune cell subpopulation ratios and functions in aged SLOs.

A tSNE plot showing subclusters of T cell subsets in young and old LNs. B Chart showing the cell ratios of subclusters of T cell subsets in young and old LNs. C UMAP plot showing subclusters of T cell subsets in young and old spleen. D Chart showing the cell ratios of subclusters of T cell subsets in young and old spleen. E, F Normalised Enrichment Score (NES) of enrichment for GO biological process terms and KEGG terms with an age-related change. Only top 15 GO or KEGG terms with P-value < 0.05 were listed for major T cell subsets. G Flow cytometric analysis of naïve CD4⁺ T cells (CD45⁺, CD3⁺, CD4⁺, CD62L⁺, CD44^-), TCM cells (CD45⁺, CD3⁺, CD4⁺, CD62L⁺, CD44⁺), TEM cells (CD45⁺, CD3⁺, CD4⁺, CD62L^-, CD44⁺) was performed. The percentage representation of the populations was shown in young and old LNs (n = 5 mice/group). H Flow cytometric analysis of CD4⁺ CTLs (CD4⁺, GZMB⁺), CD8⁺ CTLs (CD8a ⁺, GZMB⁺), Tex cells (CD45⁺, CD3⁺, CD4⁺, PD-1⁺, TIM3⁺), Tfh cells (CD45⁺, CD3⁺, CD4⁺, CD154⁺, CXCR5⁺) and Th1 cells (CD45⁺, CD3⁺, CD4⁺, CXCR3⁺) was performed. The percentage of the populations was shown in young and old LNs (n = 5 mice/group). I Flow cytometric analysis of naïve CD4⁺ T cells, TCM cells, TEM cells, naïve CD8⁺ T cells (CD45⁺, CD3⁺, CD8a⁺, CD62L⁺, CD44^-), TCM cells and TEM cells was performed. The percentage of the populations was shown in young and old spleen (n = 5 mice/group). J Flow cytometric analysis of Treg cells (CD4⁺, FOXP3⁺) and Tfh cells was performed. The percentage of the populations was shown in young and old spleen (n = 5 mice/group). P-values were calculated between two groups using an unpaired t-test. ns, not significant; *P < 0.05; **P < 0.01; ***P < 0.001.