Fig. 5: FSTL3 directly binds to TfR1 on colon cancer cells to promote VM and migration by inducing VE-Cadherin expression. | Cell Death & Disease

Fig. 5: FSTL3 directly binds to TfR1 on colon cancer cells to promote VM and migration by inducing VE-Cadherin expression.

From: Cancer-associated fibroblasts expressing FSTL3 promote vasculogenic mimicry formation and drive colon cancer malignancy

Fig. 5

A Silver staining of FSTL3-binding proteins following CO-IP. B Venn diagram showing the overlap between FSTL3-pulled down proteins, membrane proteins, and proteins associated with the HIF-1 signaling pathway. C The LC-MS/MS images of TfR1. D CO-IP followed by western blot analyses demonstrating the interaction between FSTL3 and TfR1. E Colocalization of FSTL3 and TfR1 by IF staining in HCT116 cells treated with 300 ng/mL exogenous rhFSTL3. The histogram below illustrated the change in fluorescent intensity along the line drawn in the merged images; Scale bar =10 μm. F Representative images of tube formation, cell migration, and cell invasion in vitro; Scale bar =100 μm, n = 3. G, H Quantification of tube formation (G) and migration or invasion ability of HCT116 cells (H), n = 3. I, J Western blot images (I) and quantification (J) illustrating protein expression levels in the TfR1/AKT/mTOR pathways following TfR1 knockout in HCT116 cells. All statistics are expressed as mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001; and ns, no significance. Statistical significance was calculated by one-way ANOVA with the Tukey post hoc test.

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