Fig. 3: Vasculogenic mimicry phenotype decreases cell area on stiff ECM, which is rescued by increasing pHi in metastatic lung carcinoma. | Cell Death & Disease

Fig. 3: Vasculogenic mimicry phenotype decreases cell area on stiff ECM, which is rescued by increasing pHi in metastatic lung carcinoma.

From: Intracellular pH dynamics respond to extracellular matrix stiffening and mediate vasculogenic mimicry through β-catenin

Fig. 3

a Representative images of H1299 cells plated on soft (0.5% PEGDA) and stiff (4% PEGDA) HA gels and stiff (4% PEGDA) with raised pHi. Images show differential interference contrast (DIC), Hoechst 33342 (DNA, cyan) and live-cell membrane dye (CellMask, magenta). Scale bars: 50 μm. b Quantification of single-cell area collected as shown in (a) (N = 3 biological replicates; n = 1061, soft; n = 954, stiff; n = 1078, stiff high pHi. Red lines show medians ± IQR).

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