Fig. 5: Stabilization of β-catenin abundance under high pHi rescues VM on a stiff matrix. | Cell Death & Disease

Fig. 5: Stabilization of β-catenin abundance under high pHi rescues VM on a stiff matrix.

From: Intracellular pH dynamics respond to extracellular matrix stiffening and mediate vasculogenic mimicry through β-catenin

Fig. 5

a Quantification of pHi data from parental H1299 cells and H1299 cells treated with CHIR treatment with and without treatment with sodium bicarbonate (Bicarb.) (see methods) (N = 3 biological replicates; n = 191, soft; n = 219, stiff; n = 198, stiff high pHi; n = 302, soft +CHIR; n = 373, stiff +CHIR; n = 408, stiff high pHi +CHIR. Red lines show means ± SEM). b Representative images of H1299 cells plated on stiff HA gels (4% PEGDA) with and without raised pHi (Bicarb.) and with or without CHIR treatment, fixed and stained for β-catenin. β-catenin staining is pseudocolored according to scale. Scale bars: 50 μm. c Quantification of whole cell β-catenin intensity collected as shown in (b). (N = 3 biological replicates, n = 452, stiff; n = 486, stiff high pHi; n = 415, stiff high pHi +CHIR. Red lines show medians ± IQR). d Representative images of H1299 cells plated on stiff HA gels (4% PEGDA) with and without raised pHi (Bicarb.) and with or without CHIR treatment. Images show differential interference contrast (DIC), Hoechst 33342 (DNA, cyan) and live-cell membrane stain (CellMask, magenta). Scale bars: 50 μm. e Quantification of single-cell area collected as shown in (d) (N = 3 biological replicates; n = 452, stiff; n = 486, stiff high pHi; n = 415, stiff high pHi +CHIR. Red lines show medians ± IQR).

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