Fig. 6: Low pHi is sufficient to induce vasculogenic mimicry on a soft ECM. | Cell Death & Disease

Fig. 6: Low pHi is sufficient to induce vasculogenic mimicry on a soft ECM.

From: Intracellular pH dynamics respond to extracellular matrix stiffening and mediate vasculogenic mimicry through β-catenin

Fig. 6

a Quantification of pHi data from parental H1299 cells and H1299 cells where NHE1 knockout via CRISPR (H1299-NHE1 K.O.). with and without treatment with sodium bicarbonate (Bicarb.) (see methods) (N = 3 biological replicates. Red lines show means ± SEM). b Representative images of H1299 cells plated on soft HA gels (0.5% PEGDA) with and without lowered pHi (NHE1 K.O.) and with or without increased pHi (NHE1 K.O. Bicarb.). Images show differential interference contrast (DIC) and Hoechst 33342 (DNA, cyan). Scale bars: 100 μm. c Representative images of H1299 cells plated on soft HA gels (0.5% PEGDA) with and without lowered pHi (NHE1 K.O.) and with or without increased pHi (NHE1 K.O. Bicarb.). Images show differential interference contrast (DIC) and live-cell membrane stain (CellMask, magenta). Scale bars: 50 μm. d Quantification of single-cell area collected as shown in (a) (N = 3 biological replicates; n = 383, parental; n = 267, NHE1 K.O.; n = 315, NHE1 K.O. Bicarb. Red lines show medians ± IQR).

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