Fig. 1: Effect of IFN-γ + TNF-α-treatment on the expression of ligands for NK cell-activating receptors, death receptors TRAIL-R2 and FAS, and the adhesion molecule ICAM-1 in BC cell lines. | Cell Death & Disease

Fig. 1: Effect of IFN-γ + TNF-α-treatment on the expression of ligands for NK cell-activating receptors, death receptors TRAIL-R2 and FAS, and the adhesion molecule ICAM-1 in BC cell lines.

From: Combined IFN-γ and TNF-α treatment enhances the susceptibility of breast cancer cells and spheroids to Natural Killer cell-mediated killing

Fig. 1

MCF-7 (A), MDA-MB-231 (B), and MDA-MB-468 (C) BC cell lines were untreated or treated for 24 h with non-toxic low doses of IFN-γ + TNF-α and then evaluated for surface expression of MHC class I and the indicated molecules by flow cytometry. Each panel shows a representative (left) and a summary of four independent experiments (right). Black or red lines (dashed for isotype control antibodies, solid for specific antibodies) are displayed for untreated or IFN-γ + TNF-α-treated BC cell lines, respectively (left). Normalized mean of fluorescence (MFI) for each experiment was displayed by comparing the IFN-γ + TNF-α-treated BC cell lines (red dots) with the untreated or control (black dots) cell lines (right). Mean ± SD; *p < 0.05, **p < 0.01,***p < 0.001; p value, two tailed unpaired Student-t test.

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