Fig. 6: HNF4A plays an essential role in maintaining epithelial cell polarity.

A The RNA-seq analysis showed the differentially expressed gene in MKN74 cell lines with HNF4A overexpression. The most significantly upregulated genes were displayed in the volcano map. B The gene expression correlation analysis showed the top 40 genes co-expressed with HNF4A in the TCGA_STAD cohort. The genes marked with blue asterisks were also significantly induced by HNF4A in the volcano plot. C The potential downstream target genes of HNF4A were obtained by taking the intersection of the gene most significantly induced by HNF4A (log2FC > 1) and the gene most significantly co expressed with HNF4A (R > 0.5). D The potential downstream target genes of HNF4A were verified in MKN74 cell lines with or without HNF4A overexpression using qRT-PCR assay. E The tissue-specific expression analysis of HNF4A and its downstream target genes. The expression data (RPKM value) of HNF4A and its downstream target genes in various human tissues was available from the HPA project using the NCBI website. The dot plot is generated using relative expression value of each serine protease. In short, the relative expression value of each gene was obtained by making the sum of the RPKM value of each gene in all tissues be 1. F The 35 downstream target genes induced by HNF4A (log2FC > 1) according to the RNA-seq and expression correlation analysis were collected to conducted GO analysis. G, H Analysis of the expression patterns of HNF4A and VIM in early gastric cancer patients with positive Helicobacter pylori infection based on continuous pathological sections. The images in H were a threefold enlarged display of the blue box in the images of G. **P < 0.01.