Fig. 3: Chchd2 knockout mice have normal OXPHOS activity but present mild alterations in tissue composition and morphology. | Cell Death & Disease

Fig. 3: Chchd2 knockout mice have normal OXPHOS activity but present mild alterations in tissue composition and morphology.

From: The CHCHD2-CHCHD10 protein complex is modulated by mitochondrial dysfunction and alters lipid homeostasis in the mouse brain

Fig. 3: Chchd2 knockout mice have normal OXPHOS activity but present mild alterations in tissue composition and morphology.

A Respiratory chain enzyme activity assays of CI, CI+CIII, CII, CII+CIII, and CIV in isolated mitochondria from brain, heart, and skeletal muscle (quadriceps) of Chchd2–/–and Chchd2+/+ male mice at the age of 12 months. Data are represented as means ± SEM.; n = 5; ns not significant. Quantification of B mtDNA copy number (Nd1 and Nd6/18S rRNA) and C mtDNA transcripts (Mt-Co1, CytB and Nd1) performed by qPCR in brain, heart, and skeletal muscle. Data are represented as means ± SEM; n ≥ 11; ns not significant. D Mthfd2 gene expression measured by qPCR in heart and skeletal muscle of male mice at 6 and 12 months of age. Data are represented as means ± SEM; n ≥ 4; *p < 0.05; E, F Representative electron microscopy images of tissue and mitochondrial morphology (Scale bar: 5, 1 μm and 500 nm) and sample-wise quantification of vacuoles in the heart of male mice at 12 months of age. G, H Representative electron microscopy images of tissue and mitochondrial morphology (Scale bar: 2 and 1 μm) and sample-wise quantification of sarcomere alterations in the skeletal muscle of male mice at the age of 12 months. Data are represented as means ± SEM; n = 5; *p < 0.05.

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