Fig. 5: C3G deletion in hepatocytes increases glucagon response, decreases insulin signaling, and improves glucose tolerance.
From: Deletion of C3G in hepatocytes impairs full liver maturation and alters glucose homeostasis
A Western blot analysis of P-PKA substrates in hepatocytes from C3GKOAlb and wt mice stimulated with glucagon for 5, 10, 15, 30, and 60 min, or maintained untreated normalized with β-Actin. B Western blot analysis of P-p38 and P-p70S6K (P(Thr385)-p70S6K) in hepatocytes from C3GKOAlb and wt mice stimulated with glucagon for 5, 10, 15, and 30 min, or maintained untreated normalized with β-Actin. C Western blot analysis of P-IRTyr1345, Pro-IRβ, IRβ, P-IRS1Ser307, P-Akt, Akt, P-ERKs, and ERKs in hepatocytes from C3GKOAlb and wt mice stimulated with insulin for 5, 10, and 15 min, or maintained untreated normalized with β-Actin. D Glucose tolerant test (GTT). Glucose was administered to 16 h-fasted mice, and blood glucose was measured at different time points. *p ≤ 0.05 compared to wt mice (n = 2–6).
