Fig. 4: AAV‑mediated restoration of IDE reduces α‑syn deposition in A53T α‑syn mice.

A Experimental timeline showing model establishment, AAV-IDE or control AAV-Vector administration, and behavioral testing. B Representative immunoblots of IDE and α-syn in SN and STR (n = 6 per group). C, D Quantification of IDE protein and mRNA in SN and STR tissues of WT + Vector, A53T + Vector, and A53T + IDE mice (n = 6 per group). ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05, ns not significant; ANOVA analyses and Tukey’s test for post hoc comparisons. E, F Quantification of α-syn protein and mRNA in SN and STR across the same groups (n = 6 per group). ****P < 0.0001, ***P < 0.001, **P < 0.01, ns not significant; ANOVA analyses and Tukey’s test for post hoc comparisons.