Fig. 4: Desmin deficiency results in defects in the proper expression and localization of the cardiac markers α-actinin and desmoplakin during iCMs maturation and cardiac embryonic development. | Cell Death & Disease

Fig. 4: Desmin deficiency results in defects in the proper expression and localization of the cardiac markers α-actinin and desmoplakin during iCMs maturation and cardiac embryonic development.

From: Molecular insights into the role of desmin intermediate filament network in chromatin landscape, cardiomyocyte differentiation, and maturation

Fig. 4

A Immunofluorescence staining for α-actinin in iCMDes+/+ and iCMDes−/− cultures fixed 21 days after GMT transduction (red arrows indicating ‘high’ α-actinin expression, green arrows indicating ‘medium’ α-actinin expression, yellow arrows indicating ‘low’ α-actinin expression). n = 3. Green staining: α-actinin. DAPI: blue staining of nuclei. Scale bars: 50 μm. B Immunofluorescence staining for α-actinin in iCMDes+/+ and iCMDes−/− cultures fixed 5 weeks after GMT transduction. n = 3, (red arrows indicate intercalated disks, IDs). Green staining: a-actinin. DAPI: blue staining of nuclei. Scale bars: 100 μm. C Immunofluorescence staining for desmoplakin in iCMDes+/+ and iCMDes−/− cultures fixed 14 weeks after GMT transduction. n = 3, Scale bars: 100 μm. D Western blot analysis of whole-cell lysates from co-cultures of iCMs+CF grown up to 1-month, 2-months and 3-months after GMT transduction. n = 3. E Western blot analysis of whole-cell lysates from cardiac tissue of embryonic days E11.5, E12.5, E14.5 and E15.5. n = 3 (Two biological replicates are shown with the corresponding housekeeping protein for normalization.). iCMs induced cardiomyocytes.

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